David J. Pagliarini, Ph.D.

Professor and BJC Investigator, Departments of Cell Biology and Physiology; Biochemistry and Molecular Biophysics; and Genetics

Office: (314) 273-2331; Lab: (314) 273-2330;  pagliarini [at] wustl [dot] edu


Research Interests: We are an interdisciplinary team of scientists driven to understand the biochemical underpinnings of mitochondrial dysfunction in human diseases. Together, we integrate large-scale methodologies with traditional biochemistry to investigate the modulation, adaptation, and basic metabolic function of mitochondria.

Professional Education

  • 2005-2009 Postdoctoral Fellow, Harvard Medical School
  • 2002-2005 Ph.D. Biomolecular Sciences, UC San Diego
  • 1999-2002 M.S. Biological Chemistry, University of Michigan
  • 1995-1999 B.S. Biochemistry, University of Notre Dame


Selected Recent Publications

Pptc7 is an essential phosphatase that promotes mitochondrial metabolism by enabling protein import and processing
Niemi NM, Wilson GM, Overmyer KA, Vögtle FN, Lohman DC, Schueler KL, Attie AD, Meisinger C, Coon JJ, and Pagliarini DJ
Nature Communications, 2019 10: 3197  

An isoprene lipid binding protein promotes eukaryotic coenzyme Q biosynthesis
Lohman DC*, Aydin D*, Von Bank H, Smith R, Linke V, Weisenhorn EM, McDevitt MT, Hutchins PD, Wilkerson EM, Wancewicz B, Russell JD, Stefely MS, Beebe ET, Jochem A, Coon JJ, Bingman CA, Dal Peraro M†, and Pagliarini DJ†
Molecular Cell, 2019 73(4):763-774

Multi-omics reveal specific targets of the RNA-binding protein Puf3p and its orchestration of mitochondrial biogenesis
Lapointe CP*, Stefely JA*, Jochem A, Hutchins PD, Wilson GM, Kwiecien NW, Coon JJ, Wickens MP†, and Pagliarini DJ† 
Cell Systems, 2018 6(1):125-135

Conserved lipid and small molecule modulation of COQ8 reveals regulation of the ancient kinase-like UbiB family 
Reidenbach AG, Kemmerer ZA, Aydin D, Jochem A, McDevitt MT, Hutchins PD, Stark JL, Stefely JA, Reddy T, Hebert AS, Wilkerson EM, JA, Johnson IE, Bingman CA, Markley JL, Coon JJ, Dal Peraro M, and Pagliarini DJ 
Cell Chemical Biology, 2018 25(2):154-165
Multi-omic mitoprotease profiling defines a role for Oct1p in coenzyme Q production 
Veling MT, Reidenbach AG, Freiberger EC, Kwiecien NW, Hutchins PD, Drahnak MJ, Jochem A, Ulbrich A, Rush JPR, Russell JD, Coon JJ, and Pagliarini DJ 
Molecular Cell, 2017 68(5):970-977

Mitochondrial protein functions elucidated by global mass spectrometry profiling
Stefely JA*, Kwiecien NW*, Freiberger E, Richards AL, Jochem A, Rush MJP, Ulbrich A, Robinson KP, Hutchins PD, Veling MT, Guo X, Kemmerer ZA, Connors KJ, Trujillo EA, Sokol J, Westphall MW, Hebert AS, Pagliarini DJ†, and Coon JJ†
Nature Biotechnology, 2016 34(11):1191-1197

Iron Deprivation Induces Transcriptional Regulation of Mitochondrial Biogenesis 
Rensvold JW, Krautkramer KA, Dowell JA, Denu JM, and Pagliarini DJ
Journal of Biological Chemistry, 2016 291(40):20827-20837

Mitochondrial protein interaction mapping identifies new regulators of respiratory chain function
Floyd BJ*, Wilkerson EM*, Veling MT*, Minogue CE*, Xia C, Beebe ET, Wrobel, RL, Cho H, Kremer LS, Alston CL, Gromek KA, Dolan BK, Ulbrich A, Stefely JA, Bohl SL, Werner KM, Jochem A, Westphall MS, Rensvold JW, Taylor RW, Prokisch H, Kim JJ, Coon JJ, and Pagliarini DJ
Molecular Cell, 2016 63(4): 621-32

Cerebellar Ataxia and Coenzyme Q Deficiency Through Loss of Unorthodox Kinase Activity
Stefely JA*, Licitra F*, Laredj L, Reidenbach AG, Kemmerer AZ, Grangeray A, Jaeg-Ehret T, Minogue CE, Ulbrich A, Hutchins PD, Wilkerson EM, Ruan Z, Aydin D, Hebert AS, Guo X, Freiberger EC, Reutenauer L, Jochem A, Chergova M, Johnson IE, Lohman DC, Rush MJP, Kwiecien NW, Singh PK, Schlagowski AI, Floyd BJ, Forsman U, Sindelar P, Westphall MS, Pierell F, Zoll J, Dal Peraro M, Kannan N, Bingman CA Coon JJ, Isope P, Puccio H†, and Pagliarini DJ†
Molecular Cell, 2016 63(4):608-20

Mitochondrial ADCK3 employs an atypical protein kinase-like fold to enable coenzyme Q biosynthesis 
Stefely JA*, Reidenbach AG*, Ulbrich A, Oruganty K, Floyd BJ, Jochem A, Saunders JM, Johnson IE, Minogue CE, Wrobel RL, Barber GE, Lee D, Li S, Kannan N, Coon JJ, Bingman CA, and Pagliarini DJ, 
Molecular Cell, 2015 57(1): 83-94